Skip to main content
. Author manuscript; available in PMC: 2023 Jun 1.
Published in final edited form as: Nat Immunol. 2022 Oct 31;23(12):1763–1776. doi: 10.1038/s41590-022-01343-7

Extended Data Fig. 5. KLF2 and its target genes are aberrantly expressed in NCOR1/2-deficient B cells.

Extended Data Fig. 5

a. RNA expression of Klf2 and its target genes Cd69 and Sell (CD62L; L-selectin) in wildtype (WT), NCOR1-knockout (KO) and NCOR1/2-knockout (DKO) B cells. Asterisk (*; P<0.05) indicates statistically significant expression changes compared to wildtype cells for Klf2 in PreBCRi-II (DKO; P=4.24E-15), preBCRi-III (DKO; P=1.43E-9), and immature B (DKO; P=1.34 E-8), for Cd69 in PreBCRi-II (DKO; P=1.86E-39), PreBCRi-III (DKO; P=2.74E-8), Kappa Pre B (DKO; P=1.47E-37), Lambda Pre B-I (DKO; P=1.69E-12), Lambda Pre B-III (DKO; P=9.14E-14), and Immature B (DKO; P=1.89E-7). b. Gene activity of Klf2 and its target genes Cd69 and Sell (CD62L; L-selectin)in WT, KO and DKO B cells. Asterisk (*; P< 0.05) indicates statistically significant accessibility changes compared to wildtype cells for Klf2 in Pro B VDJ (KO; P=1.23E-9), Cycling Pre B (KO; P=2.94E-36, DKO; P=4.97E-24), Kappa/Lambda Pre B (KO; P=5.31E-33), Immature B (KO; P=1.22E-14, DKO; P=6.40E-11), mature B (KO; P=4.94E-13), plasma cells (KO; P=4.94E-13, DKO; P=5.39E-7) and for Cd69 in Cycling Pro B (KO; P=1.74E-6, DKO; P=2.12E-12), Pro B VDJ (KO; P=4.20E-13, DKO; P=2.47E-30), Cycling Pre B (KO; P=1.29E-27, DKO; P=6.87E-84), Kappa/Lambda Pre B(KO; P=1.96E-57, DKO; P=6.92E-46), Immature B (KO; P=3.72E-27, DKO; P=3.13E-22), mature B (KO; P=1.68E-20, DKO;P=5.39E-7), plasma cells (KO; P=1.68E-20, DKO; P=5.39E-7) and for Sell in Kappa/Lambda Pre B (DKO; P=4.27E-7), and immature B (DKO;P=1.47E-16). c. CD62L expression of different B cell development stages in wildtype (WT) and NCOR1/2-knockout (DKO) B cells. Top represents representative flow cytometry plots for CD62L expression and bottom represents summarized frequency of CD62L+ cells for prepro B (B220+CD43+CD24−CD19−, n=4), proB/preB (B220+CD43+CD24+CD19+, n=4), small pre B (B220+CD43+CD19+IgM−, n=4), immature B (B220lowCD43+gM+, n=4), and mature B cells (B220highCD43+gM+, n=4) from three independent experiments. A two-tailed t-test was used to calculate statistical significance. Center of measure indicates mean and error bars indicate standard deviation.