FIG. 2.

(A) Total RNA was obtained from the AP1 strain at early logarithmic growth phase (EL), late logarithmic growth phase (LL), and early stationary phase (ES). RNA was subjected to Northern blotting using a probe hybridizing with sclA. A part of the gel was stained with ethidium bromide to visualize rRNA bands, used as molecular weight markers. (B) Total RNA from AP1, BMJ71, and BMJ71pMGA.1 was prepared from bacteria in early logarithmic growth phase and subjected to Northern blotting using the same probe as for panel A. (C) Representation of the putative Mga-binding element located at −107 to −147 bp from the start codon of sclA. Putative −35 and −10 boxes are indicated.