FIG. 4.

(A) Insertion-duplication mutagenesis was used to delete the part of sclA encoding the membrane-spanning M domain and the COOH-terminal part of the W domain in strain AP1 to generate the SclA⁻ strain. (B) Growth media from AP1 and SclA⁻ were TCA precipitated, and proteins were subjected to SDS-PAGE (12% acrylamide gels; reducing conditions). One gel was stained with Coomassie brilliant blue (STAIN), and two replicas were subjected to Western blotting (BLOT) using SclA antiserum or preimmune serum (dilution, 1:1,000), respectively.