Skip to main content
. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Gastroenterology. 2022 Sep 13;164(1):89–102. doi: 10.1053/j.gastro.2022.09.006

Figure 5. Stat3 inhibitor attenuates intestinal fibrosis induced by Th17 cells.

Figure 5.

CBir1 Th17 cell were transferred into Tcrβxδ−/− mice (n=4/group). Mice were injected with or without Stat3 inhibitor (10 mg/kg, HJC0152) every other day starting from the day of cell transfer. (A) Disease severity was measured by histopathology and pathological scores. (B) TNF-α and (C) IL-6 levels in supernatants of colonic organ cultures were measured by ELISA. (D) Colon tissues were stained with Sirius Red, and Collagen layer thickness were analyzed. (E-F) Col1a1 and Col6a1 levels in colonic tissues were measured by RT-PCR. (G-H) Colon tissues were stained with immunofluorescence. Collagen I thickness (G) and αSMA layer thickness (H) were measured. Representative data from 2 independent experiments with similar results. (A) Mann–Whitney U test; (B-H) unpaired Student’s t-test. *p < .05; **p < .01.