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. Author manuscript; available in PMC: 2023 Dec 12.
Published in final edited form as: Cancer Cell. 2022 Oct 27;40(12):1566–1582.e10. doi: 10.1016/j.ccell.2022.10.004

KEY RESOURCES TABLE

REAGENT or RESOUCE SOUCE IDENTIFIER
Antibodies
Rabbit anti-IGF2BP1 antibody Cell Signaling Technology Cat.: #8482, clone D33A2
Rabbit anti-IGF2BP2 antibody Cell Signaling Technology Cat.: #14672, clone D4R2F
Rabbit anti-IGF2BP3 antibody Bethyl Laboratories Cat.: A303-426A, RRID: AB_10951696
GPT2 (G-7) mouse mAb Santa Cruz Biotechnology Cat.: sc-398383
c-Myc (D3N8F) Rabbit mAb Cell Signaling Technology Cat# 13987, RRID:AB_2631168
ASCT2 Rabbit mAb Cell Signaling Technology Cat# 8057S, clone D7C12
Asct2 Rabbit mAb Cell Signaling Technology Cat# 5345S, clone V501
beta-Actin (8H10D10) Mouse mAb Cell Signaling Technology Cat# 3700, RRID:AB_2242334
GAPDH antibody (0411) Santa Cruz Biotechnology Cat# sc-47724, RRID: AB_627678
HA-Tag Rabbit mAb Cell Signaling Technology Cat# 3724, clone C29F4
Anti-METTL14 antibody, rabbit Sigma-Aldrich HPA038002, RRID:AB_10672401
Anti-METTL3 antibody [EPR18810] Sigma Cat# ab195352
Donkey anti-rabbit IgG-HRP Santa Cruz Biotechnology Cat# sc-2077, RRID: AB_631745
Goat anti-mouse IgG-HRP Santa Cruz Biotechnology Cat# sc-2055, RRID: AB_631738
Anti-rabbit IgG (H+L), F(ab′)2 Fragment (Alexa Fluor® 488) Cell Signaling Technology Cat# 4412S
IGF2BP2 antibody GeneTex Cat# GTX33256
CD45 antibody PE BD Biosciences Cat# 555483, RRID:AB_395875
CD33 antibody FITC BD Biosciences Cat# 555626, RRID:AB_395992
PE anti-mouse/human CD11b eBioscience Cat# 101208, RRID: AB_312791
CD14 Monoclonal Antibody (61D3), APC eBioscience Cat# 17-0149-41, RRID: AB_10670627
Anti-mouse CD45.2-PE eBioscience Cat# 12-0454-82, RRID:AB_465678
Anti-Mouse CD45.1 PE eBioscience Cat# 12-0453-82, RRID:AB_465675
Anti-Mouse CD45.2 APC eBioscience Cat# 17-0454-82, RRID:AB_469400
Bacterial and Virus Strains
Stbl3 E. coli Transgene Cat# CD521-02
One Shot® Stbl3™ Chemically Competent E. coli Life Technologies Cat# C737303
5-alpha Competent E. coli Transgene Cat# CD201-02
Chemicals, Peptides, and Recombinant Proteins
Tamoxifen Sigma-Aldrich Cat# T5648
Recombinant Human IGF2BP2 protein Origene Cat# TP305673
L-Glutamine (200mM) Thenno Fisher Scientific Cat# 25030-081
13C5;15N2-glutamine Cambridge Isotope Laboratories, Inc. Cat# CNLM-1275-H-PK
MEM Non Essential Amino Acids Solution (100×) Thermo Fisher Scientifific Cat# 11-140-050
Sodium Pyruvate (100mM) Thermo Fisher Scientifific Cat# 11360-070
glucose Thermo Fisher Scientifific Cat# A2494001
Halt protease inhibitor cocktail Thermo Fisher Scientifific Cat# 78442
DPBS Thermo Fisher Scientifific Cat# 14040117
11S part of luciferase GenScript
Puromycin dihydrochloride Sigma-Aldrich Cat# P8833
G418 Thermo Fisher Scientific Cat# 11811-031
Actinomycin D Sigma-Aldrich Cat# A9415
cycloheximide Sigma-Aldrich Cat# C1988
Recombinant Human IL-6 PeproTech Cat# 200-06
Recombinant Human IL-3 PeproTech Cat# 200-03
Recombinant Human Flt3-Ligand PeproTech Cat# 300-19
Recombinant Human SCF PeproTech Cat# 300-07
Recombinant Human TPO PeproTech Cat# 300-18
Recombinant mouse IL-3 PeproTech Cat# 213-13
Insulin, human recombinant zinc solution Thermo Fisher Scientifific Cat# 12585014
Penicillin Streptomycin Thermo Fisher Scientifific Cat# 15-140-122
Recombinant mouse SCF PeproTech Cat# 250-03
Recombinant murine GM-CSF PeproTech Cat# 315-03
cOmplete™, Mini, EDTA-free Protease Inhibitor Cocktail Roche Cat# 4693159001
Amersham ECL Prime Western Blotting Detection Reagent Thermo Fisher Scientific Cat# 45-010-090
Pierce Protein A/G Magnetic Beads Thermo Fisher Scientific Cat# 88803
Wright-Giemsa Stain Polysciences Cat# 24985
Wright-Giemsa Stain/Buffer Polysciences Cat# 24984
Cycloheximide solution Sigma-Aldrich Cat# C4859
Bst 3.0 DNA Polymerase New England Biolabs Cat# M0374S
Busulfan Cayman Chemical Company Cat# 14843
XF 1.0 M Glucose Solution Seahorse Bioscience Cat# 103577
XF 100 mM Pyruvate Solution Seahorse Bioscience Cat# 103578
XF 200 mM Glutamine Solution Seahorse Bioscience Cat# 103579
CWI1-2 TC Scientific Inc.
BTYNB Cayman Chemical Company Cat# 25623
NSC69557 NCI
DMSO Sigma Cat# D2650
ss-m6A RNA IDT
Pronase from Streptomyces griseus Roche Cat# 10165921001
Solutol HS-15 MedChem Express Cat# HY-Y1893
IGF2BP2 WT protein This study
IGF2BP2 KH4 protein This study
IGF2BP2 KH3-4 protein This study
Critical Commercial Assays
Glutamine/Glutamate-Glo Assay Promega Cat# J8021
EastepTM Super Total RNA Extraction Kit Promega Cat# LS1040
Seahorse XF Cell Mito Stress Test Kit Seahorse Bioscience Cat# 103015-100
ClonExpress Ultra One Step Cloning Kit Vazyme Cat# C115-02
In-Fusion HD Cloning Plus CE Takara Cat# 638916
CD117 MicroBead Kit, mouse Miltenyi Biotec Cat# 130-091-224
Maxima SYBR Green qPCR Master Mix ThermoFisher Cat# K0253
ApoSENSOR ATP Cell Viability Bioluminescence Assay Kit BioVision Cat# K254
X-tremeGENE HP DNA Transfection Reagent Roche Cat# 6366236001
FITC Annexin V Apoptosis Detection Kit I BD Biosciences Cat# 556547
MTT Cell Proliferation and Cytotoxicity Assay Kit Beyotime Cat# C0009M
CD34 MicroBead Kit, human Miltenyi Biotec Cat# 130-046-702
Lineage Cell Depletion Kit Miltenyi Biotec Cat# 130-090-858
HiScript III RT SuperMix for qPCR (+gDNA wiper) Vazyme Biotech Cat# R323-01
QIAGEN Plasmid Mini Kit QIAGEN Cat# 12125
Mammalian Protein Purification System Promega Cat# G6790
Protein Thermal Shift kit ThennoFisher Cat# 4462263
Epi™ Ribosome Profiling Kit Epibiotek Cat# R1814
RNA clean&ConcentratorTM-5 kit Zymo Cat# R1016
EpiTM RiboRNA Depletion Kit Epibiotek Cat# R1805
QIAseq miRNA Library kit QIAGEN Cat# 1103679
VAHTS Stranded mRNA-seq Library Prep Kit for Illumina V2 Vazyme Biotech Cat# NR612-02
Deposit Data
RNA-seq (Raw and analyzed data) This study GSE199204
Ribo-seq (Raw and analyzed data) This study GSE211618
Experimental Models: Cell lines
HEK293T ATCC CRL-3216
Molm13 DSMZ ACC-554 RRID: CVCL_2119
U937 ATCC CRL-1593.2
MonoMac 6 DSMZ ACC-124
NB4 DSMZ ACC-207
THP1 ATCC TIB-202; RRID: CVCL_0006
ML2 DSMZ ACC-15
Kasumi-1 ATCC CRL-2724; RRID: CVCL_0589
C1498 ATCC TIB-49
SUP-B15 ATCC CRL-1929
HL60 ATCC CCL-240
Nomo-1 DSMZ (ACC-542); RRID:CVCL_1609
MV4-11 ATCC (CRL-9591); RRID:CVCL_0064
Experimental Models: Organisms/Strains
NRGS mouse The Jackson Laboratory Stock No: 024099
C57BL/6 mice Harlan Laboratories Stock No: 057
C57BL/6 mice GemPharmatech Co. Ltd Stock No: N000013
B6.SJL-PtprcaPepcb/BoyCrCrl mice Charles River Laboratories Strain code: 564
Mettl14fl/flCREERT From Dr. Chuan He
Igf2bp2 fl/fl The Jackson Laboratory Stock No: 032494
SD rat SPF (Beijing) Biotechnology Co., Ltd.
Recombinant DNA
pCDH-IGF2BP2-HA This study N/A
pCDH-Igf2bp2-HA This study N/A
pCDH-IGF2BP2-KH3-4 This study N/A
MSCV-PIG--IGF2BP2-HA This study N/A
MSCV-PIG-Igf2bp2-HA This study N/A
pLKO.1-shIGF2BP2-1 This study N/A
pLKO.1-shIGF2BP2-2 This study N/A
pLKO.1-shIGF2BP2-3 This study N/A
pLKO.1-shIgf2bp2-1 This study N/A
pLKO.1-shIgf2bp2-2 This study N/A
pLKO.1-shGPT2-1 This study N/A
pLKO.1-shGPT2-2 This study N/A
pLKO.1-shSLC1A5-1 This study N/A
pLKO.1-shSLC1A5-2 This study N/A
pLKO.1-shMYC-1 This study N/A
pLKO.1-shMYC-2 This study N/A
pLKO.1-shGpt2-1 This study N/A
pLKO.1-shGpt2-2 This study N/A
pLKO.1-shSlc1a5-1 This study N/A
pLKO.1-shSlc1a5-2 This study N/A
pLKO.1-shMyc-1 This study N/A
pLKO.1-shMyc-2 This study N/A
pLKO.1-shYTHDF2 This study N/A
pLKO.1-shPABPC1-1 This study N/A
pLKO.1-shPABPC1-2 This study N/A
pCDH-HA-GPT2 This study N/A
pCDH-HA-SLC1A5 This study N/A
MSCV-ERT2-IRES-GFP This study N/A
MSCV-Cre-ERT2-IRES-GFP This study N/A
psi-LVRU6MH- shIGF2BP2-3 This study N/A
pSIN4- IGF2BP2 This study N/A
pSIN4- IGF2BP2-KH3-4 This study N/A
Oligonucleotides
DNA primers or RNA oligos, see Table S2 Integrated DNA Technologies (IDT)
Software and Algorithms
Bowtie2-2.3.2 53 http://bowtie-bio.sourceforge.net/bowtie2/index.shtml
Cufflinks-2.2.1 54 http://cole-trapnell-lab.github.io/cufflinks/
Homer-4.8 55 http://homer.ucsd.edu/homer/
MACS2-2.2.7.1 56 https://github.com/taoliu/MACS
RSEM-1.2.31 57 https://deweylab.github.io/RSEM/
samtools-1.3.1 58 http://samtools.sourceforge.net/
STAR-2.7.2b 59 http://code.google.com/p/rna-star/
jvenn −1.6.x 60 http://jvenn.toulouse.inra.fr/app/index.html
Bedtools-2.25.0 61 http://bedtools.readthedocs.io/en/latest/
DESeq2-1.32.0 62 http://www.bioconductor.org/packages/release/bioc/html/DESeq2.html
Cutadapt (version 3.4) http://code.google.com/p/cutadapt/
R-3.3.3 https://www.r-project.org/
phantompeakqualtools 63 http://encodeproject.org/ENCODE/deeptools.ie-freiburg.mpg.de
Deeptools (version 3.5.1) 64
igv-2.3.72g 65 http://software.broadinstitute.org/software/igv/
GSEA-2.2.3 66 http://software.broadinstitute.org/gsea/index.jsp
MetaboAnalystR R package-2.0 67 https://github.com/xia-lab/MetaboAnalystR
ELDA 68 http://bioinf.wehi.edu.au/software/elda/
X-tile 52 https://medicine.yale.edu/lab/rimm/research/software/
Maven (v8.1.27.11) http://maven.princeton.edu/index.php