Table 3.
Optimization procedure using RSM to maximize phenolic content and/or antioxidant activity and/or anti-elastase activity.
| BSH extract | Optimal conditions of RSM model | Predicted response values | ||||
|---|---|---|---|---|---|---|
| Temperature (°C) | Propanediol content (%) | Time (min) | Phenolic content (mg GAE/L) | Antoxidant activity H-ORACFL (mmol TE/L) | Anti-elastase activity (% inhibition)a | |
| 14 | 75 | 98.75 | 77.91 | 4700 | 78.07 | 100.0 |
| 15 | 75 | 100.0 | 60.60 | 4660 | 75.58 | 100.0 |
| 16 | 74 | 79.92 | 51.45 | 3940 | 81.90 | 100.0 |
| 17 | 75 | 98.64 | 77.87 | 4696 | 78.13 | 100.0 |
| 18 | 74 | 79.92 | 51.45 | 3940 | 81.90 | 100.0 |
Bold values shows optimized variable(s) for each extract.
aThe effective or inhibitory concentration that reduces the enzyme activity to 50% (EC50 value) of the control EGCG is 2.3 mg/L (experimental data, n = 3).