TABLE 1.
Kinetic values of the glucosyltransferase reaction of α551 for UDP-glucose and UDP–N-acetylglucosamine
| Nucleotide-sugar | Kinetic valuesa
|
||||
|---|---|---|---|---|---|
| Km (μM) | Relative Km (%) | kcat (h−1) | Relative kcat (%) | Relative kcat/Km (%) | |
| UDP–N-acetylglucosamine | 17 | 100 | 2,800 | 100 | 100 |
| UDP-glucose | 307 | 5.5 | 282 | 10.0 | 0.6 |
a
Values were all determined to be statistically accurate to a standard deviation of not greater than ±20%. The kinetic parameters were determined from Lineweaver-Burk plots of initial rates of reaction as a function of the UDP-glucose or UDP–N-acetylglucosamine concentration. Assays were performed at a fixed GST-Rac concentration.