Table 3.
Kinetic parameters for the equilibrium compulsory ordered bi bi mechanism (Equation 2).a
[Substrate] on abscissa |
Substrate binds first |
kcat (s−1) | KP5C (μM) | KNADH (μM) | Adj. R2 | AIC |
---|---|---|---|---|---|---|
L-P5C | L-P5C | 2300± 130 | 1500± 700 | 27 ± 12 | 0.95806 | −280 |
NADH | NADH | 1400 ± 130 | 215 ± 75 | 79 ± 29 | 0.97033 | −282 |
Measured at pH 7.5 and 26°C.