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. Author manuscript; available in PMC: 2024 Jan 1.
Published in final edited form as: Arch Biochem Biophys. 2022 Nov 19;733:109468. doi: 10.1016/j.abb.2022.109468

Table 3.

Kinetic parameters for the equilibrium compulsory ordered bi bi mechanism (Equation 2).a

[Substrate]
on abscissa
Substrate
binds first
kcat (s−1) KP5C (μM) KNADH (μM) Adj. R2 AIC
L-P5C L-P5C 2300± 130 1500± 700 27 ± 12 0.95806 −280
NADH NADH 1400 ± 130 215 ± 75 79 ± 29 0.97033 −282
a

Measured at pH 7.5 and 26°C.