. Author manuscript; available in PMC: 2024 Jan 1.

Published in final edited form as: Arch Biochem Biophys. 2022 Nov 19;733:109468. doi: 10.1016/j.abb.2022.109468

Table 4.

Kinetic parameters for the steady-state compulsory ordered bi bi mechanism (Equation 3).^a

[Substrate] on abscissa	Substrate binds first	k_cat (S⁻¹)	K^P5C (μM)	K^NADH (μM)	$K_{m}^{P 5 C} (μ M)$	$K_{m}^{N A D H} (μ M)$	Adj. R²
L-P5C	L-P5C	3326 ± 314	110 ± 70	N/A	267 ± 64	117 ± 26	0.98133
NADH	NADH	2171 ± 296	N/A	31 ± 14	257 ± 66	67 ± 19	0.98672

Measured at pH 7.5 and 26°C.