Table 1.
Reagents and resources.
| REAGENT | SOURCE | IDENTIFIER | City | Country |
|---|---|---|---|---|
|
| ||||
| Biotinylated Goat-Anti-Mouse | Biolegend | 405303 | San Diego | CA |
| Mouse-Anti-Human CD3 (Clone, OKT3) | Bioxcell | Be0001-2 | Lebanon | NH |
| Mouse-Anti-Human CD28 (Clone, 9.3) | Bioxcell | Be0248 | Lebanon | NH |
| Leukopak | New York Blood Center | New York | NY | |
| Acrylamide (40%) | Sigma-Aldrich | 01697 | St. Louis | MO |
| Bis-Acrylamide (2%) | Fisher Scientific | BP1404-250 | Hampton | NH |
| Ammonium Persulfate (APS) | Sigma-Aldrich | A3678 | St. Louis | MO |
| Tetramethylethylenediamine (TEMED) | Sigma-Aldrich | T7024 | St. Louis | MO |
| Streptavidin-Acrylamide | ThermoFisher | S21379 | Waltham | MA |
| Blebbistatin | STEMCELL | 72402 | Vancouver | Canada |
| AICAR | Cell Signaling Technology | 9944 | Danvers | MA |
| Oligomycin | MilliporeSigma | O4876 | Burlington | MA |
| Alexa Fluor 488 anti-human FOXP3 Antibody (Clone, 206D) | BioLegend | 320111 | San Diego | CA |
| CD25 Antibody, anti-human, REAfinity APC (Clone, REA570) | Miltenyi Biotec | 130-113-284 | Bergisch Gladbach | Germany |
| CD3 Mouse anti-Human, Alexa Fluor 488, Clone OKT3 | eBioscience | 50-112-9566 | Waltham | MA |
| MitoTracker Red CMXRos | ThermoFisher | M7512 | Waltham | MA |
| RPMI 1640 | ThermoFisher | 21870092 | Waltham | MA |
| HEPES Buffer | ThermoFisher | 15630 | Waltham | MA |
| L-Glutamine | ThermoFisher | 25030 | Waltham | MA |
| Fetal Bovine Serum (FBS) | ThermoFisher | Gibco-Performance 26140-079 | Waltham | MA |
| β -Mercaptoethanol | Sigma-Aldrich | M3148 | St. Louis | MO |
| Penicillin-Streptomycin | ThermoFisher | 15140122 | Waltham | MA |
| Ficoll-Paque Plus | Cytiva | 17144002 | Marlborough | MA |
| CD4+CD25+Regulatory T Cell Isolation Kit | Miltenyi Biotec | 130-091-301 | Bergisch Gladbach | Germany |
| IL-2 | PeproTech | 200-02 | East Windsor | NJ |
| TGF- β1 | PeproTech | 100-21C | East Windsor | NJ |
| Sodium Pyruvate (100 Mm) | ThermoFisher | 11360070 | Waltham | MA |
| MEM Non-Essential Amino Acids Solution (100X) | ThermoFisher | 11140050 | Waltham | MA |
| True-Nuclear™ Transcription Factor Buffer Set | BioLegend | 424401 | San Diego | CA |
| Dynabeads | ThermoFisher | 11132D | Waltham | MA |
| CSFE | ThermoFisher | C34554 | Waltham | MA |
| FITC anti-human CD127 antibody (Clone, A019D5) | Biolegend | 351312 | San Diego | CA |
| BV421 anti-human CD4 antibody (Clone, OKT4) | Biolegend | 317434 | San Diego | CA |