Fig. 3. Epileptic variants did not affect the side-chain structure of GLT-1.

A The three epileptic variants were mutated with amino acids with similar properties as the original amino acids (G82A, G82E, G82I, G82K; L85A, L85G; P289A, P289E, P289I, P289K). B After transfection with different plasmids, D-[³H]-Asp uptake activity was determined. n = 3 per group. The representative blots and quantification of total proteins (C, D), biotinylated membrane proteins (E, F), and non-biotinylated proteins (G, H) of GLT-1 were measured by Western blot. n = 3 per group. Results are expressed as mean ± SD. ^**P < 0.01 vs. WT. Statistical significance was determined using one-way ANOVA and Tukey’s tests for post hoc comparisons.