Fig. 4. Epileptic variants affected the STIM1/Orai1 complex.

A, B The representative blots and quantification of STIM1 after transfection with different plasmids (SK, WT, G82R, L85P, G82R/L85P, and P289R) in HeLa cells were determined by Western blot. n = 3 per group. C, D The representative blots and quantification of the phosphorylation of CaMKII and total CaMKII after transfection with different plasmids in HeLa cells were determined by Western blot. n = 3 per group. E, F The interaction between GLT-1 and STIM1 or Orai1 was determined by a Co-IP assay using GLT-1 as the capture antibody. G, H The interaction between GLT-1 and STIM1 or Orai1 was determined by a Co-IP assay using STIM1 as the capture antibody. n = 3 per group. Results are expressed as mean ± SD. ^**P < 0.01 vs. WT. Statistical significance was determined using one-way ANOVA and Tukey’s tests for post hoc comparisons.