Fig. 6. Establishment of G82R/L85P variant knock-in mice.

A, B Strategy of establishing the G82R/L85P variant knock-in mice. C, D Representative blots and quantification of GLT-1 in the hippocampus of WT and TG mice were determined by Western blot. n = 4 per group. E, F The representative blots and quantification of STIM1, Orai1, phosphorylation of CaMKII, and total CaMKII in the hippocampus of WT and TG mice were determined by Western blot. n = 4 per group. G The representative trace mice traveled in the open field. The total traveled distance (H), movement speed (I) in the OFT, and duration in the center zone of open field (J) are shown. K, L The total traveled distance and movement speed in the EPM test. M The grasping test was used to examine the grip strength of mice. N The rotarod test was used to examine the motor coordination of mice. O The pole-climbing test was used to examine bradykinesia in mice. n = 5 for WT mice (four males and one female) and n = 4 for TG mice (three males and one female). Results are expressed as mean ± SD. ^**P < 0.01, ^**P < 0.05 vs. WT. Statistical significance was determined using Student’s t test.