TABLE 1.
Strains and plasmids used in this study
| Strain or plasmid | Description | Reference or source |
|---|---|---|
| E. coli strains | ||
| SM10 λ pir | thi thr leu tonA lacY supE recA::RP-4-Tc::Mu (λ pir) | 42 |
| SC625 | TOP10 TnFC-rfbT mutant; Cmr | This study |
| V. cholerae strains | ||
| E7946 | El Tor; Ogawa | |
| Peru-2 | C6709 ΔattRS1; Inaba | 60 |
| Peru-15 | C6709 ΔattRS1 recA::htpG-ctxB; spontaneously nonmotile; Inaba | 33 |
| NM3 | Peru-2 flgE::TnFC-rfbT Fla−; Ogawa | This study |
| NM11 | Peru-2 flgE::TnFC-rfbT Fla−; Ogawa | This study |
| SC631 | NM3 ΔCmr; Ogawa | This study |
| SC632 | NM11 ΔCmr; Ogawa | This study |
| Plasmids | ||
| pBR322 | Standard cloning vector; Apr | New England Biolabs |
| pCP20 | FLP recombinase expression plasmid; pSC101 origin of replication; Apr | 8 |
| pCR2.1 | Cloning vector; Apr | Invitrogen |
| pNEB193 | Standard cloning vector; Apr | New England Biolabs |
| pSC121.1 | rfbT gene from E7946 cloned into pCR2.1; Apr | This study |
| pSC138 | TnFC-rfbT; Cmr | This study |
| pSC141 | Replicon fusion of pCP20 and pBR322; Apr Tcr | This study |