Table II.
Phospholipids present in YF1*7.1/β2m renatured in folding buffer only and in folding buffer to which E. coli total lipid extract was added
| Phospholipid | Mass Spectrum Values | |||||
|---|---|---|---|---|---|---|
| Molecule | Fatty Acid Chain | Mass (Da) | Sample (main AUC) | Control (main AUC) | Sample/ Control Ratio | Retention Time (min) |
| Renatured in folding buffer only | ||||||
| LPE | 17:1 | 465.286 | 4.09E+05 | 8.33E+02 | 491 | 1.80 |
| PE | 17:1/16:0 | 703.515 | 2.99E+05 | 9.30E+03 | 32 | 9.98 |
| PE | 16:0/18:1 | 717.531 | 1.21E+05 | 2.98E+03 | 40 | 10.32 |
| LPE | 19:1 | 493.317 | 9.63E+04 | 3.56E+02 | 270 | 2.70 |
| PE | 16:0/16:1 | 689.5 | 8.97E+04 | 8.41E+02 | 107 | 9.32 |
| Renatured in folding buffer with E. coli total lipid extract added | ||||||
| LPE | 18:1 | 479.30 | 4.61E+06 | 7.70E+02 | 5980 | 2.08 |
| LPG | 17:1 | 496.28 | 3.03E+06 | 8.15E+03 | 372 | 1.36 |
| LPE | 17:1 | 465.29 | 1.51E+06 | 4.79E+03 | 316 | 1.67 |
| LPE | 16:1 | 451.27 | 1.00E+06 | 5.04E+03 | 199 | 1.43 |
| LPG | 16:0 | 484.28 | 7.57E+05 | 6.53E+02 | 1160 | 1.45 |
Sample refers to YF1*7.1 and the β2m heterodimer fraction. Control refers to the β2m alone fraction. AUC, area under the control; LPE, lyso-PE; LPG, lyso-PG.