Table 4.
Reagents and resources.
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| BsaI-HFv2 | New England BioLabs | Cat#R3733L |
| T4 DNA Ligase | New England BioLabs | Cat#M0202L |
| Adenosine-5 Triphosphate (ATP) | New England BioLabs | Cat#P0756S |
| Lipofectamine 2000 | Thermo Fisher Scientific | Cat#11668019 |
| T5 Exonuclease | New England BioLabs | Cat#M0663L |
| Tris Base (1 M, pH > 10.0) | Boston Bioproducts | Cat#BB-2686 |
| TrypLE | Thermo Fisher Scientific | Cat#12605010 |
| Puromycin Dihydrochloride | Thermo Fisher Scientific | Cat#A1113803 |
| Blasticidin S HCl | Thermo Fisher Scientific | Cat#A1113903 |
| Penicillin-Streptomycin | Thermo Fisher Scientific | Cat#15070063 |
| Gibco DMEM, high glucose, pyruvate | Thermo Fisher Scientific | Cat#11995040 |
| Opti-MEM I Reduced Serum Medium | Thermo Fisher Scientific | Cat#31985062 |
| Embryonic Stem Cell FBS | Thermo Fisher Scientific | Cat#16141002 |
| Nuclease-free H2O | Invitrogen | Cat#10977015 |
| HiFi Hot Start - Readymix | KAPA Biosystems | Cat#KK2602 |
| Q5 High-Fidelity Master Mix | New England BioLabs | Cat#M0492L |
| NEBNext Ultra II Q5 Master Mix | New England BioLabs | Cat#M0544L |
| QIAquick PCR Purification Kit | QIAGEN | Cat#28106 |
| Sodium Acetate Solution (3 M), pH 5.2 | Thermo Fisher Scientific | Cat#R1181 |
| Monarch PCR & DNA CleanUp Kit | New England BioLabs | Cat#T1030S |
| Monarch DNA Gel Extraction Kit | New England BioLabs | Cat#T1020S |
| NucleoSpin Gel and PCR Cleanup | Macherey-Nagel | Cat#74609.50 |
| QIAGEN Plasmid Plus Midi Kit | QIAGEN | Cat#12943 |
| Invitrogen 1% E-GELS EX | Invitrogen Corporation | Cat#G402021 |
| NEB 5-alpha F̀Iq Competent E. coli | New England BioLabs | Cat#C2992H |
| HEK293T | ATCC | Cat#CRL-3216 |
| pCMV-PEmax-P2A-MLH1dn | Addgene | Cat#174828 |