FIGURE 3.

IPA analysis of differentially expressed transcripts in high‐risk versus low‐risk RPE EVs. Ingenuity IPA analysis of differential transcriptomics results indicate that EVs may be involved in disease related mechanisms in high‐risk AMD RPE, including oxidative stress response, angiogenesis, actin cytoskeleton signaling, protein homeostasis and senescence. (A) Statistically significantly enriched pathways against the inferred activation z‐score. (B) IPA graphical summaries representing networks of the major pathways identified as the most significant in the differential transcriptomics data for the apical and basal high‐risk compared to low‐risk RPE EVs. The edges show direct (solid lines) and indirect (dashed lines) interactions between molecules in the network. Inferred edges are shown with dotted lines. Orange colour—predicted activation, blue colour—predicted inhibition state. Interactions are inferred from using the Ingenuity knowledge base.