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. 2022 Dec 14;22:19–29. doi: 10.1016/j.reth.2022.11.005

Fig. 5.

Fig. 5

The effects of CDKN2B-AS1/miR-122-5p/P4HA1 axis on the viability and migration of thyroid cancer cells.(A–D) RT-qPCR was used to detect the transfection efficiency of miR-122-5p inhibitor (I) and shRNA-targeted P4HA1 (sh-P4HA1), with U6 and GAPDH serving as internal controls.(E–F) After 24 or 48 h of co-culture of exosomes and recipient cells, the effect of CDKN2B-AS1/miR-122-5p/P4HA1 on cell viability was assessed by CCK-8 assay.(G–H) Cell migration in each group was measured by wound healing experiment. ∗∗∗p < 0.001 vs. IC (inhibitor control) + sh-NC; ###p < 0.001 vs. IC + sh-P4HA1; ˆˆˆp < 0.001 vs. I + sh-NC; &p < 0.05, &&p < 0.01, &&&p < 0.001 vs. control (No transfection); θp < 0.05, θθθp < 0.001 vs. sh-CDKN2B-AS1 (CSCs) (transfected into CSCs); △p < 0.05, △△p < 0.01, △△△p < 0.001 vs. I (transfected into recipient thyroid cancer cells); †p < 0.05, ††p < 0.01, †††p < 0.001 vs. sh-P4HA1 (transfected into recipient thyroid cancer cells).