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. 2022 Dec 8;13:1057583. doi: 10.3389/fphar.2022.1057583

FIGURE 4.

FIGURE 4

Hif1a positively regulated Ptgs2 by binding to its promoter region. (A) Veen digraph of candidate transcription factors determined by AnimalTFDB 3.0 intersecting with ferroptosis driver. Nine candidates were screened out. (B) Potential motifs of the nine candidates. (C–E) Hif1a expression following CME detected by RT-qPCR (n = 6) and western blotting (n = 4). (F) The expression level of Hif1a detected by RT-qPCR (n = 6). (G,H) The expression of Hif1a protein determined by western blotting (n = 4). (I,J) The expression of Ptgs2 determined by western blotting (n = 4) and RT-qPCR, respectively (n = 6). (K–M) ChIP-qPCR assay showing the interaction of Hif1a and four segments (P1-P4) of Ptgs2 promoter (n = 3). IgG was a negative control. GAPDH served as an internal control was performed to quantitatively normalized the protein data. Data are presented as the normalized mean ± SEM (to sham, shNC, or IgG). Values in shams, shNCs or IgGs were averaged and normalized to 1 (C–M). *p < 0.05. **p < 0.01. Hif1a: hypoxia-inducible factor 1 subunit alpha; Ptgs2: prostaglandin-endoperoxide synthase-2; ChIP: Chromatin immunoprecipitation.