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. 2022 Oct 14;15(2):307–325. doi: 10.1016/j.jcmgh.2022.10.005

Figure 2.

Figure 2

HDLBP knockdown inhibits the proliferation of HCC cells in vitro and in vivo.A, A CCK-8 assay was performed in Hep3B and SNU387 cells transfected with the Flag tag vector control (Flag-CTL) or Flag tag plasmid containing the HDLBP sequence (Flag-HBP) at the indicated time points. B, A colony formation assay was performed in transfected cells for 2 weeks. Representative images (left panel) and the relative number of colonies (right panel) are shown. C, A CCK-8 assay was performed in Hep3B and SNU387 cells infected with the HDLBP overexpression lentivirus (Lv-HBP) or control lentivirus (Lv-CTL) at the indicated time points. D, A colony formation assay was performed in infected cells for 2 weeks. Representative images (left panel) and the relative number of colonies (right panel) are shown. E, A CCK-8 assay was performed in Huh7 and HepG2 cells transfected with the control siRNA (CTL-si) or HDLBP siRNAs (HBP-si) at the indicated time points. F, A colony formation assay was performed in transfected cells for 2 weeks. Representative images (left panel) and the relative number of colonies (right panel) are shown. G, A CCK-8 assay was performed in Huh7 and HepG2 cells transfected with the control shRNA (CTL-sh) or HDLBP shRNAs (HBP-sh) at the indicated time points. H, A colony formation assay was performed in transfected cells for 2 weeks. Representative images (left panel) and the relative number of colonies (right panel) are shown. I–K, Huh7 cells were stably infected with a lentivirus containing the CTL-sg or HBP-sg sequence. Transfected cells were injected subcutaneously into the right flanks of male BALB/c nude mice (n = 5 mice per group). Representative images (I) of xenografts are displayed, and tumor weights (J) and volumes (K) were measured. L, Representative images of hematoxylin and eosin staining and IHC staining for HDLBP and Ki67 expression in serial sections. For all the experiments described above, the data are presented as the means ± standard deviations (SDs), and 3 independent experiments (N = 3) were performed in triplicate. ∗P < .05; ∗∗P < .01; and ∗∗∗P < .001.