Table 4.
Bacterial strains, plasmids, and primers used in this study
| Bacterial strains, plasmids, or primers | Description | Reference |
|---|---|---|
| Bacterial strains | ||
| Escherichia coli DH5α | F−, φ 80dlacZ∆M15, ∆(lacZYA-argF)U169, deoR, recA1, endA1, hsdR17(rK−, mK+), phoA, supE44, λ−, thi-1, gyrA96, relA1 | [49] |
| Bacillus subtilis 168 | trpC2 | [50] |
| Lacticaseibacillus rhamnosus GG | ATCCa 53,103 | Purchased from ATCC |
| Plasmids | ||
| pUB19 | E. coli-B. subtilis shuttle vector, Apr, Kmr | [51] |
| pUB19-cotG-p40 | Spore display of p40 using the CotG anchor | This study |
| Primers | ||
| CotG-F | 5′-CCCTTCGACGCGTbCAGCTGGC-3′ | This study |
| CotG-R |
5′-ACTTGTGTCGCTTCCTCCTCCTCCTTTGTATTTC TTTTTGACTACCCAGCAATTGCCGTC-3′ |
This study |
| p40-F |
5′-TACAAAGGAGGAGGAGGAAGCGACACAAGTG CCAGCATCGCATCTAACAAGAGCG-3′ |
This study |
| p40-R |
5′- AAGGAAAAAGCGGCCG†CAAAAGGAAAATTA CCGGTGGATGTAAACGTAGCTGCTGGC-3’ |
This study |
aATCC, American type culture collection (Manassas, VA, USA)
bBold letters indicate restriction sites