Figure 1.

The efficacy of immunotherapy in EGFR-mutated lung tumors. (A) Schematic diagram depicting treatment schedule for EGFR-mutated tumors. After confirmation of developing lung tumor nodules, the mice were randomly allocated to two groups. Red arrow indicating the PD-1 blockade was administered 200 µg/mouse by intraperitoneal injection every 3 days and blue arrows indicating the IgG control was administered 200 µg/mouse by intraperitoneal injection every 3 days. Mouse were sacrificed for tumor analysis at indicated time. (B) Representative lung images of H&E staining in each group at indicated time point. Scale bars, 2.5 mm. (C) Analysis of lungs weight in each group at day 5 and day 11. (D) Analysis of tumor invasion areas in each group at Day 5 and Day 11. n=3–5 mice per group at indicated time point. Unpaired Student’s t-test was performed and results in each group were presented as mean±SEM. *p<0.05, **p<0.01, ***p<0.001, and ns represents p values with no statistical difference.