Figure 3.

Glial channels and transporters needed for chemotaxis are required for the function of Amsh cells

(A–F) Calcium transients generated in Amsh by perfusion with octanol (1:1,000, yellow shaded area) as measured by % increase of GCaMP-6s fluorescence above the baseline (ΔF/F₀) in wild type and mutants with reduced octanol avoidance kqt-2, egl-36, kcc-1, best-9, and ent-4 for the first stimulation (black) and second stimulation (red) (left panels). The right panels show the peak percentage of GCaMP-6s (ΔF/F₀). n is shown within each left panel.

(G) Average time to peak in seconds of octanol induced Ca²⁺ transients for the indicated strains.

(H) The relative to wild type basal calcium levels of Amsh glia for the indicated strains. For g and h, the n is indicated in the columns.

(I–L) Calcium transients generated in Amsh by perfusion with isoamyl alcohol (1:100, cyan shaded area) as measured by % increase of GCaMP-6s fluorescence above the baseline (ΔF/F₀) in wild type, and mutants with reduced isoamyl alcohol avoidance egl-36, kcc-1, and clh-3 for the first stimulation (black) and second stimulation (red). The right panels show the peak percentage of GCaMP-6s ΔF/F₀. n is shown in each left panel.

(M) Average time to peak in seconds of isoamyl alcohol induced Ca²⁺ transients for the indicated strains.

(N) The relative to wild type basal calcium levels of Amsh glia for the indicated strains. The n is indicated in the columns. Data are shown as mean ± SE. p values are shown in the panels and were obtained by unpaired Student’s t-test or in the case of panels g h, m, and n by ANOVA with Tukey’s correction.