Figure 4.

ASH neuronal function in channel and transporter mutants

Calcium transients generated in ASH neurons by perfusion with octanol (1:1,000, yellow shaded area) as measured by % increase of GCaMP-6s fluorescence above the baseline (ΔF/F₀) in (A) wild type and mutants with reduced octanol avoidance and related glia specific rescue strains as follows, (B) kqt-2,(C) kqt-2 glia rescue,(D) egl-36,(E) egl-36 glia rescue, (F) ent-4, (G) kcc-1,(H) human KCC1 glia rescue in kcc-1mutant,(I) worm kcc-1 glia rescue in kcc-1mutant, (J) best-9, and (K) best-9 glia rescue. The first stimulation is in black and second stimulation is in red. The right panels show the peak percentage of GCaMP-6s (ΔF/F₀). N is shown each left panel. (L) Time to peak in seconds of octanol induced Ca²⁺ transients for the indicated strains. The n is sown in each column. Data are shown as mean ± SE. p values are shown in the panels and were obtained by unpaired Student’s t-test or in the case of panel L, by ANOVA with Tukey’s correction by comparing each mutant with WT. Rescues were compared with the corresponding mutant by t-test.