Figure 6.

AWC neuronal activity in response to diluted isoamyl alcohol in channel and transporter mutants

(A) Calcium transients generated in AWC neurons by perfusion with isoamyl alcohol (1:1,000, cyan shaded area) as measured by % increase of GCaMP5 fluorescence above the baseline (ΔF/F₀) in wild type and egl-36, egl-36 glia rescue worms, kcc-1, human KCC1 glia rescue strain, worm kcc-1 glia rescue worms, clh-3, and clh-3 glia rescue worms for the first stimulation (A-D) and the second stimulation (E-H). The bar graphs show the peak percentage of GCaMP-6s (ΔF/F₀upon odorant perfusion (ON) and odorant removal (OFF). The number of neurons tested is shown in the graphs.

(I) Time it takes for the fluorescence to return to F₀ after odorant removal during the first exposure to isoamyl alcohol for wild type, mutants, and related glia specific rescue strains.

(J) The relative to wild type basal calcium in AWC neurons of the indicated strains which have reduced attraction to isoamyl alcohol and their rescue strains: egl-36, egl-36 glia rescue, kcc-1, clh-3, and clh-3 glia rescue. N is indicated in each column. Data are shown as mean ± SE. p values are shown in the panels and were obtained by unpaired Student’s t-test or in the case of panels I and J, by ANOVA with Tukey’s correction, comparing each mutant with WT. Rescues were compared with the corresponding mutant by t-test.