Figure 7.

Glial channels and transporters are needed for the function of AWC neurons in response to concentrated isoamyl alcohol

(A) Calcium transients generated in AWC neurons by perfusion with isoamyl alcohol (1:100, cyan shaded area) as measured by % increase of GCaMP5 fluorescence above the baseline (ΔF/F₀) in wild type egl-36, egl-36 glia rescue, kcc-1, human KCC1 glia rescue, worm kcc-1 glia rescue, clh-3, and clh-3 glia rescue for the first stimulation (A–D) and the second stimulation (E–H). The bar graphs show the peak percentage of GCaMP5 (ΔF/F₀) upon odorant perfusion (ON) and odorant removal (OFF). The number of neurons tested is shown in the graphs. Data are shown as mean ± SE. p values are shown in the panels and were obtained by unpaired Student’s t-test.