REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
Escherichia coli	CGC	OP50
Chemicals, peptides, and recombinant proteins
Octanol	Sigma-Aldrich	Cat#112615
Glycerol	Sigma-Aldrich	Cat#G5516
Isoamyl alcohol	Sigma-Aldrich	Cat#AX1440
Na-acetate	Sigma-Aldrich	Cat#S2889
Diacetyl	Sigma-Aldrich	Cat#B0682
Ethyl alcohol	Pharmco	Cat#111000200
Chloroform	Sigma-Aldrich	Cat#C2432
Dimethyl sulfoxide	Sigma-Aldrich	Cat#D5879
Bicuculline	VWR	Cat#TBC1890
α-MDG	Sigma-Aldrich	Cat#102987-308
Nifedipine	Thomas Scientific	Cat#C931M79
KCl	Sigma-Aldrich	Cat#9541
NaN3	Sigma-Aldrich	Cat#S2002
Gluture	Fisher Scientific	Cat#NC0632797
Experimental models: Organisms/strains
N2	CGC81	N2
clh-3(ok768)	CGC82	RB905
egl-36(n728n398)	CGC83	KP1008
ent-4(ok2161)	CGC82	VC1934
glc-3(ok321)	CGC82	RB594
glt-5(bz70)	CGC84	ZB1099
glt-5(ok1987)	CGC82	RB1615
gtl-1(ok375)	CGC82	VC244
kcc-1(ok648)	CGC82	VC476
kcc-1(ok692)	CGC82	VC542
tax-2(p691)	CGC36	PR691
kqt-2(ok732)	CGC82	RB883
nsls109 [F16F9.3p::DTA(G53E) +unc-122p::GFP]	CGC16	OS2248
odr-3(n2150)	CGC85	CX2205
kyIs722 [str-2p::GCaMP5(D380Y) + elt-2::mCherry]	CGC	CX17256
kqt-2(tm642)	NBRP	N/A
best-9(tm7876)	NBRP	N/A
Ex[PT02B11.3::abts-4 RNAi + unc-122p::GFP + Pvap-1::RFP]	This study	BLC340
Ex[PT02B11.3::best-9 RNAi + unc-122p::GFP + Pvap-1::RFP]	This study	BLC342
Ex[PT02B11.3::clh-3 RNAi + unc-122p::GFP + Pvap-1::RFP]	This study	BLC337
Ex[PT02B11.3::egl-36 RNAi + unc-122p::GFP + Pvap-1::RFP]	This study	BLC336
Ex[PT02B11.3::glc-3 RNAi + unc-122p::GFP + Pvap-1::RFP]	This study	BLC345
Ex[PT02B11.3::kcc-1 RNAi + unc-122p::GFP + Pvap-1::RFP]	This study	BLC329
Ex[PT02B11.3::twk-21 RNAi + unc-122p::GFP + Pvap-1::RFP]	This study	BLC350
Ex[PT02B11.3::twk-33 RNAi + unc-122p::GFP + Pvap-1::RFP]	This study	BLC338
Ex[PT02B11.3:kqt-2 RNAi + unc-122p::GFP + Pvap-1::RFP]	This study	BLC358
Ex[pSra-6::GCaMP-6s + unc-122::GFP]	This study	BLC400
clh-3(ok768);Ex[PT02B11.3::clh-3b cDNA + Punc-122::GFP]	This study	BLC356
kcc-1(ok648);Ex[PT02B11.3::SLC12A4 + Punc-122::GFP]	This study	BLC547
best-9(tm7876);Ex[PT02B11.3::best-9 cDNA + Punc-122::GFP]	This study	BLC551
egl-36(n728n398);Ex[PT02B11.3::egl-36 cDNA + Punc-122::GFP]	This study	BLC552
ent-4(ok2161);Ex[PT02B11.3::ent-4 cDNA + Punc-122::GFP]	This study	BLC555
kqt-2(ok732);Ex[PT02B11.3::kqt-2 cDNA + Punc-122::GFP]	This study	BLC491
Ex[PT02B11.3::unc-25 RNAi + unc-122p::GFP]	This study	BLC500
best-9(tm7876);[osm-10::GFP + lin-15(+)]	This study	BLC554
clh-3(ok768); [gcy-5p::GFP + lin-15(+)]	This study	BLC553
clh-3(ok768);[osm-10::GFP + lin-15(+)]	This study	BLC548
clh-3(ok768);[str-2::GFP + lin-15(+)]	This study	BLC550
egl-36(n728n398);[osm-10::GFP + lin-15(+)]	This study	BLC556
egl-36(n728n398);[str-2::GFP + lin-15(+)]	This study	BLC560
ent-4(ok2161);[osm-10::GFP + lin-15(+)]	This study	BLC557
kcc-1(ok648);[osm-10::GFP + lin-15(+)]	This study	BLC558
kcc-1(ok648);[str-2::GFP + lin-15(+)]	This study	BLC561
kqt-2(ok732);[osm-10::GFP + lin-15(+)]	This study	BLC559
best-9(tm7876);Ex[PT02B11.3::GCaMP6s + Punc-122::GFP]	This study	BLC562
clh-3(ok768);Ex[PT02B11.3::GCaMP6s + Punc-122::GFP]	This study	BLC563
egl-36(n728n398);Ex[PT02B11.3::GCaMP6s + Punc-122::GFP]	This study	BLC564
ent-4(ok2161);Ex[PT02B11.3::GCaMP6s + Punc-122::GFP]	This study	BLC566
kcc-1(ok648);Ex[PT02B11.3::GCaMP6s + Punc-122::GFP]	This study	BLC565
kqt-2(ok732);Ex[PT02B11.3::GCaMP6s + Punc-122::GFP]	This study	BLC567
best-9(tm7876);Ex[PT02B11.3::GCaMP6s + Punc-122::GFP]	This study	BLC568
egl-36(n728n398);Ex[pSra-6::GCaMP-6s + unc-122::GFP]	This study	BLC570
ent-4(ok2161);Ex[pSra-6::GCaMP-6s + unc-122::GFP]	This study	BLC572
kcc-1(ok648);Ex[pSra-6::GCaMP-6s + unc-122::GFP]	This study	BLC573
kqt-2(ok732);Ex[pSra-6::GCaMP-6s + unc-122::GFP]	This study	BLC 492
best-9(tm7876);Ex[pSra-6::GCaMP-6s + unc-122::GFP]	This study	BLC572
egl-36(n728n398);Ex[pSra-6::GCaMP-6s + unc-122::GFP]	This study	BLC577
ent-4(ok2161);Ex[pSra-6::GCaMP-6s + unc-122::GFP]	This study	BLC578
kcc-1(ok648);Ex[pSra-6::GCaMP-6s + unc-122::GFP]	This study	BLC579
clh-3(ok768);[str-2p::GCaMP5(D380Y) + elt-2::mCherry]	This study	BLC574
egl-36(n728n398);[str-2p::GCaMP5(D380Y) + elt-2::mCherry]	This study	BLC524
kcc-1(ok648);[str-2p::GCaMP5(D380Y) + elt-2::mCherry]	This study	BLC575
clh-3(ok768);Ex[PT02B11.3::clh-3b cDNA + Punc-122::GFP];[str-2p::GCaMP5(D380Y) + elt-2::mCherry]	This study	BLC583
egl-36(n728n398);Ex[PT02B11.3::egl-36 cDNA + Punc-122::GFP];[str-2p::GCaMP5(D380Y) + elt-2::mCherry]	This study	BLC584
kcc-1(ok648);Ex[PT02B11.3::SLC12A4 + Punc-122::GFP];[str-2p::GCaMP5(D380Y) + elt-2::mCherry]	This study	BLC585
best-9(tm7876);Ex[pSra-6::GCaMP-6s + unc-122::GFP + PT02B11.3::best-9 cDNA + Pmec-4::mcherry]	This study	BLC587
egl-36(n728n398);Ex[pSra-6::GCaMP-6s + unc-122::GFP + PT02B11.3::egl-36 cDNA + Pmec-4::mcherry]	This study	BLC588
ent-4(ok2161);Ex[pSra-6::GCaMP-6s + unc-122::GFP + PT02B11.3::ent-4 cDNA Pmec-4::mcherry]	This study	BLC580
kcc-1(ok648);Ex[pSra-6::GCaMP-6s + unc-122::GFP + PT02B11.3::SLC12A4 cDNA + Pmec-4::mcherry]	This study	BLC581
kqt-2(ok732);Ex[pSra-6::GCaMP-6s + unc-122::GFP + PT02B11.3::kqt-2 cDNA + Pmec-4::mcherry]	This study	BLC582
kcc-1(ok648);Ex[pSra-6::GCaMP-6s + unc-122::GFP + PT02B11.3::kcc-1a cDNA + Pmec-4::mcherry]	This study	BLC648
kcc-1(ok648);Ex[pSra-6::GCaMP-6s + unc-122::GFP + Pstr-2::kcc-1a cDNA + Pmec-4::mcherry]	This study	BLC649
best-9(tm7876);Ex[pT02B11.3::SuperClomeleon]	This study	BLC616
kcc-1(ok648);Ex[pT02B11.3::SuperClomeleon]	This study	BLC628
clh-3(ok768);Ex[pT02B11.3::SuperClomeleon]	This study	BLC621
Ex[pT02B11.3::SuperClomeleon]	This study	BLC498
best-9(tm7876);Ex[PT02B11.3::kcc-1 RNAi + unc-122p::GFP + Pvap-1::RFP]	This study	BLC567
kqt-2(ok732);Ex[PT02B11.3::egl-36 RNAi + unc-122p::GFP + Pvap-1::RFP]	This study	BLC571
clh-3(ok768);kcc-1(ok648)	This study	BLC605
Ex[PT02B11.3:egl-19 RNAi + unc-122p::GFP]	This study	BLC630
Oligonucleotides
pPD95_75	A gift from Andrew Fire	RRID:Addgene_1494
pPD95_75 (pSra-6::::GCaMP-6s)	This study	N/A
pPD95_75 (pT02B11.3::SLC12A4)	This study	N/A
pPD95_75 (pT02B11.3::clh-3b cDNA)	This study	N/A
pPD95_75 (pT02B11.3::kcc-1a cDNA)	This study	N/A
pPD95_75 (pstr-2::kcc-1a cDNA)	This study	N/A
pPD95_75 (pT02B11.3::sense kcc-1 RNAi)	This study	N/A
pPD95_75 (pT02B11.3::antisense kcc-1 RNAi)	This study	N/A
pPD95_75 (pT02B11.3::sense abts-4 RNAi)	This study	N/A
pPD95_75 (pT02B11.3::antisense abts-4 RNAi)	This study	N/A
pPD95_75 (pT02B11.3::sense best-9 RNAi)	This study	N/A
pPD95_75 (pT02B11.3::antisense best-9 RNAi)	This study	N/A
pPD95_75 (pT02B11.3::sense clh-3 RNAi)	This study	N/A
pPD95_75 (pT02B11.3::antisense clh-3 RNAi)	This study	N/A
pPD95_75 (pT02B11.3::sense egl-36 RNAi)	This study	N/A
pPD95_75 (pT02B11.3::antisense egl-36 RNAi)	This study	N/A
pPD95_75 (pUnc-122::GFP)	(Grant et al., 2015)20	N/A
pPD95_75 (pVap-1::RFP)	(Johnson et al., 2020)22	N/A
pPD95_75 (pT02B11.3::sense glc-3 RNAi)	This study	N/A
pPD95_75 (pT02B11.3::antisense glc-3 RNAi)	This study	N/A
pPD95.75 (pT02B11.3::sense twk-33 RNAi)	This study	N/A
pPD95.75 (pT02B11.3::antisense twk-33 RNAi)	This study	N/A
pPD95_75 (pT02B11.3::SuperClomeleon)	This study	N/A
pPD95_75 (pT02B11.3::sense twk-21 RNAi)	This study	N/A
pPD95_75 (pT02B11.3::antisense twk-21 RNAi)	This study	N/A
pPD95_75 (pT02B11.3::sense kqt-2 RNAi)	This study	N/A
pPD95_75 (pT02B11.3::antisense kqt-2 RNAi)	This study	N/A
pPD95_75 (pT02B11.3::best-9 cDNA)	This study	N/A
pPD95_75 (pT02B11.3::ent-4 cDNA)	This study	N/A
pPD95_75 (pT02B11.3::kqt-2 cDNA)	This study	N/A
pMec-4::mcherry	This study	N/A
pT02B11.3::sense egl-19 RNAi	This study	N/A
pT02B11.3::antisense egl-19 RNAi	This study	N/A
Software and algorithms
Fiji (ImageJ)	NIH	http://fiji.sc
		RRID:SCR_002285
Micro-manager	Vale Lab, UCSF	https://micro-manager.org/
		RRID:SCR_000415
RStudio	RStudio	http://www.rstudio.com/
		RRID:SCR_000432
GraphPad Prism	GraphPad software	http://www.graphpad.com/
		RRID:SCR_002798