FIG. 6.

Invasive E. coli-induced tyrosine phosphorylation of FAK and its association with paxillin was blocked by overexpression of mutant forms of FAK. Confluent HBMEC transfected with GFP, Phe397 FAK, FRNK, and Arg454 FAK were infected with E44 for different periods of time (minutes); total cell lysates were prepared and immunoprecipitated with anti-FAK antibody. The immune complexes were separated by SDS-PAGE and subjected to immunoblotting with antiphosphotyrosine antibody (pY), anti-FAK antibody (FAK), or antipaxillin antibody (PAX). C, control.