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. 2022 Dec 8;13:1025422. doi: 10.3389/fpls.2022.1025422

Figure 1.

Figure 1

Effects of BABA on Fragaria vesca resistance to Botrytis cinerea infection of leaves. (A) B. cinerea infection 5 days post-inoculation in leaves of F. vesca plants after soil-drenching (left) with 0.2 mM β-aminobutyric acid (BABA) or foliar spraying with 0.3 mM BABA (right), or (B) soil-drenching with 0 mM, 0.05 mM, 0.15 mM, 0.3 mM or 0.6 mM BABA two days before inoculation. Levels of B. cinerea infection was measured as the ratio between B. cinerea and F. vesca DNA using qPCR with genome-specific primers for B. cinerea (Bc3F and Bc3R) and F. vesca (EF1αF and EF1αR). Levels of B. cinerea in untreated control plants are set to 1. ‘n’ = number of biological replicates (i.e. individual plants), and error bars show standard error ( ± 1 SE). P-values report comparisons between control plants and plants subjected BABA using Student’s t-test. ‘ns’ = not statistically significant. (C) Representative pictures of disease progression in F. vesca plants 4 days and 7 days after inoculation, n = 5. Treatment combinations: B. cinerea-infected (NBI; No-BABA and Infected), mock-infected (NBNI; No-BABA and Non-Infected), BABA-treated and B. cinerea-infected (BI; BABA and Infected), and BABA-treated and mock-infected (BNI; BABA and Non-Infected).