Table 1.
Results of the main bacterial reverse mutation test conducted using the plate incorporation method with N. crassa biomass.
| Concentration (μg/plate) |
Revertant colonies per plate (mean ± standard deviation) | |||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| TA98 | TA100 | TA1535 | TA1537 | WP2uvrA | ||||||
| –S9 | +S9 | –S9 | +S9 | –S9 | +S9 | –S9 | +S9 | –S9 | +S9 | |
| Negative controla | 20 ± 1.0 | 22 ± 3.5 | 96 ± 5.5 | 97 ± 3.5 | 15 ± 2.0 | 14 ± 2.0 | 10 ± 3.5 | 11 ± 1.0 | 35 ± 3.6 | 37 ± 1.0 |
| 1.58 | 20 ± 1.0 | 18 ± 2.1 | 95 ± 5.5 | 94 ± 2.0 | 16 ± 4.7 | 13 ± 3.8 | 10 ± 4.7 | 8 ± 2.1 | 40 ± 3.5 | 36 ± 3.6 |
| 5.0 | 22 ± 4.0 | 22 ± 2.5 | 96 ± 6.1 | 95 ± 2.6 | 15 ± 1.5 | 14 ± 3.2 | 8 ± .6 | 8 ± 3.1 | 34 ± 1.5 | 38 ± 1.5 |
| 15.8 | 23 ± 3.1 | 24 ± 5.3 | 93 ± 1.5 | 97 ± 6.1 | 17 ± 6.7 | 14 ± 1.5 | 7 ± 2.1 | 8 ± 1.5 | 38 ± 5.5 | 39 ± 2.1 |
| 50 | 22 ± 3.0 | 24 ± 1.7 | 92 ± 1.5 | 93 ± 4.9 | 15 ± 1.0 | 15 ± 3.5 | 9 ± .6 | 7 ± 1.7 | 36 ± 3.8 | 34 ± 4.5 |
| 158 | 20 ± 1.2 | 21 ± 1.0 | 92 ± 3.2 | 94 ± 2.5 | 19 ± 1.0 | 14 ± 1.5 | 9 ± 1.2 | 10 ± .6 | 36 ± 7.2 | 35 ± 1.5 |
| 500 | 19 ± 1.5 | 21 ± 2.5 | 92 ± 2.1 | 93 ± 5.1 | 13 ± 2.5 | 16 ± 6.7 | 8 ± 1.0 | 7 ± 1.5 | 36 ± 4.0 | 36 ± 4.0 |
| 1,580b | – | – | – | – | – | – | – | – | – | – |
| 5,000b | – | – | – | – | – | – | – | – | – | – |
| Positive controlc,d | 850 ± 48.2 | 4067 ± 66.7 | 622 ± 52.0 | 3379 ± 321.1 | 514 ± 14.6 | 258 ± 33.9 | 195 ± 17.6 | 507 ± 26.4 | 430 ± 18.8 | 122 ± 5.0 |
+S9 = with metabolic activation; –S9 = without metabolic activation; 2-AA = 2-aminoanthracene; MMS = methylmethanesulfonate; NaN3 = sodium azide.
a100 μL/plate sterile water.
bThe assessment of background lawn was obscured by precipitate.
cPositive controls, –S9: WP2uvrA = 2.5 μL/plate MMS; TA1537 = 8.0 μg/plate IC 191 acridine; TA98 = 6.0 μg/plate daunomycin; TA100 and TA1535 = 1.5 μg/plate NaN3.
dPositive controls, +S9: All = 10 μg/plate 2-AA.