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. 2022 Dec 8;13:1063679. doi: 10.3389/fimmu.2022.1063679

Table 1.

Test for detection of RCA in the BBV154 sample.

Sample Details Number of passages in A549 cells TCID50 assay performed on Virus titers after three passages (TCID50)
BBV154 sample alone* 3 A549 cells No CPE
BBV154 sample spiked with 1 TCID of Ad5 1010
BBV154 sample spiked with 10 TCID of Ad5 1010.35
Ad5 alone 10 TCID 1011
BBV154 sample alone HEK cells No CPE

*Hexon copies: 10.58 log copies/mL; Spike copies: 10.18 log copies/mL; virus particle by A260: 2.82 x 1012 virus particle/mL.