Table 3.
Differentially expressed genes between D21B and D21T groups (Log2fold>1 in both groups).
| Gene | Function | Nematode Host Response | D21 BOLUS vs. TRICKLE infection | D21 BOLUS infection VS. NAIVE | D21 TRICKLE infection vs NAIVE | D7 BOLUS infection vs NAIVE | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Fold Change | Adjusted p-value | Fold Change | Adjusted p-value | Fold Change | Adjusted p-value | Fold Change | Adjusted p-value | |||||
| Genes upregulated with trickle infection | ||||||||||||
| 1 | Adamts4 |
Metalloproteinase
(ECM remodelling) |
No study found | 123.96 | 7.87E-05 | <2 | >0.05 | 94.57 | 1.32E-03 | 2.88 | >0.05 | |
| 2 | Osm |
Secreted cytokine Growth regulator
(cytokine/growth factor signalling) |
No study found | 49.22 | 1.67E-02 | <2 | >0.05 | 73.15 | 1.48E-02 | <2 | >0.05 | |
| 3 | Ndc80 | Kinetochore complex component, regulated by IL-4 in resident macrophages (cell cycle & apoptosis) |
(172) | 22.04 | 1.07E-03 | -11.18 | 2.07E-02 | <2 | 4.87E-01 | -58.52 | 6.79E-04 | |
| 4 | Cxcl5 | Chemokine produced by eosinophils | (173) | 12.56 | 9.85E-04 | 13.53 | >0.05 | 169.92 | 9.56E-05 | 137.52 | 2.82E-04 | |
| 5 | S100a9 | Controls macrophage accumulation & cytokine production | (174) | 12.13 | 3.50E-04 | - <2 | >0.05 | 12.63 | 1.55E-03 | 22.21 | 1.60E-04 | |
| 6 | Nod2 | Pattern Recognition Receptor | (175) | 8.06 | 2.42E-03 | -8.76 | 2.91E-03 | <2 | >0.05 | -11.57 | 1.46E-03 | |
| 7 | S100a8 | Controls macrophage accumulation & cytokine production | (174) | 7.82 | 1.15E-02 | <2 | >0.05 | 6.86 | 3.06E-02 | 12.94 | 4.49E-03 | |
| 8 | Adamts12 | Metalloproteinase | No study found | 7.25 | 4.65E-06 | -3.72 | 1.17E-02 | <2 | >0.05 | -56.23 | 7.66E-06 | |
| 9 | Cx3cl1 | Fraktalkine, involved in fibrosis | (176) | 5.44 | 1.10E-08 | -4.51 | 1.32E-05 | <2 | >0.05 | -3.61 | 2.82E-04 | |
| 10 | Igf1 |
Hormone similar to insulin
Shales the macrophage activation phenotype |
(84) | 3.82 | 6.07E-05 | - <2 | >0.05 | 3.54 | 7.99E-04 | -2.99 | 2.01E-02 | |
| 11 | Nos2 | Inducible Nitric Oxide Synthase involved in Nitric Oxide production | (177) | 2.92 | 5.63E-03 | -21.68 | 6.70E-18 | -7.42 | 1.57E-10 | -10.77 | 3.52E-12 | |
| 12 | Smad2 | Signal transducer for the TGFbeta receptor | (178) | 2.39 | 5.50E-06 | -3.11 | 9.41E-08 | - <2 | >0.05 | -4.44 | 3.57E-12 | |
| 13 | Ifit1bl1 | IFN‐stimulated genes | (179) | 2.34 | 1.02E-03 | -5.52 | 2.71E-10 | -2.36 | 1.54E-03 | - <2 | >0.05 | |
| 14 | H2-Q10 | MHC I gene | No study found | 2.19 | 1.56E-03 | <2 | >0.05 | 3.67 | 1.14E-06 | 5.11 | 4.21E-09 | |
| 15 | Socs3 | Tumour suppressor, limiting intestinal epithelial cell proliferation | (180) | 2.17 | 4.43E-03 | -2.25 | 7.16E-03 | - <2 | >0.05 | -2.11 | 1.60E-02 | |
| 16 | Retnlb | Blocks nematode feeding | (181) | 2.14 | 8.05E-03 | 14.78 | 3.98E-18 | 31.67 | 1.40E-32 | 28.93 | 8.28E-28 | |
| 17 | Nfil3 | Non classical regulatory T cell gene | (182) | 2.08 | 4.95E-03 | <2 | >0.05 | 2.76 | 2.40E-04 | <2 | >0.05 | |
| Genes downregulated with trickle infection | ||||||||||||
| 1 | Cxcr4 | Eosinophil recruitment during Type-2 granuloma formation | (181) | 4.96 | 5.68E-04 | 22.27 | 2.82E-07 | 4.49 | 2.00E-02 | 5.04 | 1.65E-02 | |
| 2 | Ptgdr | Binding by prostaglandin D2 activates eosinophils | (183) | 4.54 | 7.90E-11 | 2.42 | 9.91E-04 | <2 | >0.05 | 4.87 | 1.73E-10 | |
| 3 | Lamb3 | Regulates cell growth, motility & adhesion. Role in wound healing. | No study found | 3.86 | 5.94E-04 | -2.42 | 4.35E-02 | -9.34 | 8.92E-09 | -5.54 | 3.87E-05 | |
| 4 | Insig1 | Regulates cholesterol biosynthesis | No study found | 2.13 | 9.96E-05 | 2.19 | 4.31E-04 | <2 | 9.41E-01 | 3.50 | 6.57E-09 | |
| 5 | Ptgs1 | Macrophage polarisation in presence of IL-4 | (184) | 2.05 | 9.51E-07 | 2.61 | 3.72E-08 | <2 | 2.56E-01 | <2 | >0.05 | |
| 6 | Kit | Essential for development and survival of mast cells | (140) | 2.01 | 4.93E-02 | 5.86 | 2.40E-05 | 2.92 | 1.50E-02 | -6.42 | 6.36E-03 | |
| 7 | Ski | TGFβ signalling | (185) | 2.00 | 5.01E-07 | 2.77 | 9.88E-10 | <2 | >0.05 | 2.27 | 2.08E-06 | |