TABLE 1.
Contents of experimental solutions.
| Compounds | aCSF (mM) | Isolation solution (mM) | aCSFH–50 (mM) | aCSFH–100 (mM) | aCSFK+ (mM) | OGD (mM) |
| NaCl | 122 | – | 98 | 67 | 75 | 122 |
| NMDG | – | 110 | – | – | – | – |
| KCl | 3 | 2.5 | 3 | 3 | 50 | 3 |
| NaHCO3 | 28 | 24.5 | 28 | 28 | 28 | 28 |
| Na2HPO4 | 1.25 | 1.25 | 1.25 | 1.25 | 1.25 | 1.25 |
| Glucose | 10 | 20 | 10 | 10 | 10 | – |
| CaCl2 | 1.5 | 0.5 | 1.5 | 1.5 | 1.5 | 1.5 |
| MgCl2 | 1.3 | 7 | 1.3 | 1.3 | 1.3 | 1.3 |
| Osmolality (mOsmol/kg) | ∼300 | ∼300 | ∼250 | ∼200 | ∼300 | ∼300 |
aCSF, artificial cerebrospinal fluid; aCSFH–50, 250 mOsmol hypotonic solution; aCSFH–100, 200 mOsmol hypotonic solution; aCSFK+, 50 mM K+ solution; NMDG, N-methyl-D-glucamine; OGD, oxygen-glucose deprivation.