TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s) | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| XL-1 Blue | recA1 lac endA1 gyrA96 thi hsdR17 supE44 rclA1 (F′ proAB lacIqlacZΔM15 Tn10) Δ(ara-leu) araD ΔlacX74 galE galK phoA20 | Pharmacia |
| CC118 λpir | thi-1 rpsE rpoB argE(Am) recA1 λpir phage lysogen | 11 |
| JCB571 | phoR mutant, zih-12::Tn10 dsbA::mini-kan Tetr | 4 |
| S. flexneri | ||
| M90TS | Wild type, serotype 5, Strr | 2 |
| Sh4 | dsbA::kan mutant, Strr Kanr | 38 |
| Sh42 | dsbA33G Strr | This study |
| Plasmids | ||
| pCVD442 | Suicide cloning vector | 11 |
| pJYU15 | Derivative of pCVD442 for delivery of the dsbA33G mutation | This study |
| pMMB66 | Broad-host-range plasmid with ptac promoter at the multiple-cloning site | 27 |
| pJYU5 | Derivative of pMMB66 expressing functional recombinant DsbAHis | This study |
| pBEJ18 | Derivative of pMMB66 expressing nonfunctional recombinant DsbA33GHis | This study |