Table 4. IC₅₀ of hCA II and hCA IX and % Inhibition^a.

	IC50 ± SEM (μM) and % inhibition
compounds	hCA II	hCA IX	hCA XII
9	0.18 ± 0.05	22.31%	37.49%
10	0.93 ± 0.05	14.58 ± 1.07	9.17 ± 0.85
11	1.18 ± 0.14	0.17 ± 0.05	2.99 ± 0.32
12	34.31%	32.14%	0.58 ± 0.06
13	32.70%	10.36 ± 1.45	37.78%
14	6.49 ± 0.63	16.13%	0.95 ± 0.08
15	1.93 ± 0.15	47.23%	1.05 ± 0.11
17	11.51 ± 0.16	1.71 ± 0.65	44.48%
18	4.17 ± 0.29	0.21 ± 0.09	1.68 ± 0.15
19	0.72 ± 0.06	1.01 ± 0.05	38.14%
20	9.73%	29.21%	40.81%
21	20.34%	45.42%	36.70%
22	0.26 ± 0.03	4.93 ± 1.83	4.29 ± 0.24
23	13.28%	28.21%	35.76%
24	1.24 ± 0.96	1.25 ± 0.02	9.90 ± 0.66
25	0.38 ± 0.01	9.76 ± 1.03	43.91%
26	4.45 ± 0.11	1.28 ± 0.09	2.02 ± 0.28
acetazolamide	1.19 ± 0.04	1.08 ± 0.02	1.55 ± 0.03

^aAll of the reported results are the mean of three independent experiments and compounds showing >50% inhibition were further analyzed for the IC₅₀ value at different dilutions. The IC₅₀ values were calculated through nonlinear regression analysis using GraphPad Prism version 8. SEM = standard error mean.