FIGURE 2.

Impaired novel object recognition and trace fear conditioning in Cox10 cKO mice. (a,b) Novel object recognition test (NORT). The Y-axes depict the preference (%); the X-axes depict the experimental groups: Control—control mice; Cox10 cKO—Aldh1l1-Cre/ERT2::Cox10^fl/+ mice. Compared to controls, Cox10 cKO mice exhibited the significantly decreased preference for a novel object. Two-way ANOVA revealed a significant effect of the genotype, F(1,55) = 19.26, p < .001. Fisher LSD post-hoc test showed that Cox10 cKO female mice exhibited significantly less preference compared to their respective controls, *denotes p < .05. (c) Compared to controls, Cox10 cKO male mice exhibited significantly decreased freezing during presentation of the cue (sound). Student’s t-test showed that Cox10 cKO males had significantly decreased freezing compared to control males (t = 3.19; p = .003), n = 22 for control; n = 9 for Cox10 cKO; *Denotes p < .05. (d) No effects on TFC were observed in Cox10 cKO female mice. (e) Compared to controls, Cox10 cKO male mice exhibited the significantly decreased freezing behavior during the inter-tone intervals. Student t-test showed that Cox10 cKO males had significantly decreased freezing compared to control males (t = 3.51; p = .001); n = 22 for control; n = 9 for Cox10 cKO, *Depicts p < .05. (f) No effects on freezing during the inter-tone intervals in Cox10 cKO female mice; n = 18 for control; n = 10 for Cox10 cKO