FIGURE 3.

Fecal sample preparation: After testing for contaminants and pathogenic species, stool samples are mixed with saline and glycerol with a final ratio of 25% stool, 65% saline, and 10% glycerol. Mixing is done through a blender or centrifugation to homogenize the sample with the adequate ratio of substrates. The mixture is then filtered and resuspended until it is fully homogenized. The homogenized solution is stored in cryotolerant containers at –80°C for FMT use. When ready for use, the solution is loaded into capsules or delivered through colonoscopy, endoscopy, enteric nasal tube or enema.