Figure 1.

Regulatory DNA elements to consider when making plant RP expression constructs. Some DNA elements are essential in expression constructs, including a promoter, the coding sequence (CDS) and terminator; however many components in expression constructs are non-essential, including scaffold/matrix attachment regions (S/MARs), enhancers, introns, tags and to some extent untranslated regions (UTRs). Each of these elements has implications in recombinant protein expression, by affecting either transcription, translation, stability or efficacy of purification. The main roles of each element are summarised in this figure and should be considered when designing RP expression constructs.