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. 2022 Dec 21;13:529. doi: 10.1186/s13287-022-03215-1

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — Cardiovascular progenitor cells play an essential role in the self-organization of a chambered cardiac organoid. A Mixed, control population stained with SIRPα and EDNRA fluorescent antibodies. SIRPα single-positive cells (bottom right quadrant) contain the cardiomyocyte population, while the SIRPα, EDNRA double-positive cells (top right quadrant) contain the cardiovascular progenitor population. B Percentages of contracting and chambered cardiac organoids generated using different compositions of cell populations. DN Double Negative Cells; CM SIRPA⁺ EDNRA⁻ Cardiomyocytes; CVP SIRPA⁺, EDNRA⁺ Cardiovascular Progenitor Cells (n = 6 per group). C Representative 2-Photon and brightfield images stitched from adjacent fields depicting CCOs generated using 50% CVPs and 50% CMs. CVPs were stained with Calcein AM (green). Scale bars, 500 µm. D Representative confocal images of CCOs generated using CVPs and CMs. CVPs were stained with Calcein AM (green) and Hoechst33342 (blue) and CMs were only stained with Hoechst33342. Scale bars, 500 µm