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. 2022 Dec 22;20:197. doi: 10.1186/s12964-022-01006-y

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© The Author(s) 2022

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Effect of NEK5 overexpression in MCF-10A cells. A Expression of NEK5-BioID-MYC vector in MCF-10A cells. Cell lysates were Western blotted with an anti-NEK5 antibody. EV- empty vector. B Effect of NEK5 overexpression on monolayer proliferation. Cells were subjected to a MTS assay. Data represent the mean and standard deviation (SD) of 5 biological replicates. C Effect of NEK5 overexpression on clonogenicity. MCF-10A NEK5 and control cells were plated at low-density and incubated for 8 d prior to colony quantification. The data represent the mean and SD of 5 replicates. *Indicates p < 0.05