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. 2000 Nov;68(11):6478–6481. doi: 10.1128/iai.68.11.6478-6481.2000

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FIG. 2 — Replication of C. pneumoniae in IFN-γ-treated ASMC and competitive inhibition of IDO activity using 1-MT. Infected ASMC were treated with increasing concentrations of IFN-γ for 48 h followed by quantitation of immunofluorescently stained inclusions (■). ASMC were pretreated with 20 mM 1-MT, infected with C. pneumoniae, and stimulated with increasing concentrations of IFN-γ (). To normalize results, data are expressed as a percentage of the number of inclusions from ASMC without IFN-γ treatment (100% is 24.8 ± 6.5 inclusions per 100 ASMC at a magnification of ×400. ∗, P < 0.05.

Inline graphic — Replication of C. pneumoniae in IFN-γ-treated ASMC and competitive inhibition of IDO activity using 1-MT. Infected ASMC were treated with increasing concentrations of IFN-γ for 48 h followed by quantitation of immunofluorescently stained inclusions (■). ASMC were pretreated with 20 mM 1-MT, infected with C. pneumoniae, and stimulated with increasing concentrations of IFN-γ (). To normalize results, data are expressed as a percentage of the number of inclusions from ASMC without IFN-γ treatment (100% is 24.8 ± 6.5 inclusions per 100 ASMC at a magnification of ×400. ∗, P < 0.05.