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. 2022 Dec 8;12:1055648. doi: 10.3389/fonc.2022.1055648

Figure 5.

Figure 5

FAM83D knockdown suppresses cell migration, invasion, and EMT in HEY and SKOV3 cells. (A) Migratory ability by scratch wound healing assay. The closure areas were quantified by comparison with the original wound area by Image J. Scale bar: 500 μm. The migration activity is expressed as mean ± SEM. *p < 0.05 vs. cells transfected with siCON cells (t-test, N = 3). (B) Representative images of migration and invasion were detected by transwell assay in SKOV3 and HEY cells transfected with siCON and siFAM83D cell groups. Scale bar: 200 μm. **p < 0.01 vs. cells transfected with siCON cells, ***p < 0.001 vs. cells transfected with siCON cells (t-test, N = 3). (C) GSEA analysis of the relationship between FAM83D and EMT. (D) The protein expression of GAPDH, E-cadherin, N-cadherin, and vimentin detected by Western blot in SKOV3 and HEY cells transfected with siCON and siFAM83D. The right panel, the level of proteins was quantified by gray analysis. *P < 0.05, ** P< 0.01 and *** P< 0.001 vs. cells transfected with siCON cells (t-test, N = 3). (E) The mRNA expression of GAPDH, E-cadherin, N-cadherin, and vimentin were detected by qRT-PCR in SKOV3 and HEY cells transfected with siCON and siFAM83D. *p < 0.05 vs. cells transfected with siCON cells, **p < 0.01 vs. cells transfected with siCON cells, ***p < 0.001 vs. cells transfected with siCON cells (t-test, N = 3).