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Pachytene stage, V. berus spermatocyte I. Immunodetection of crossing-over marker, MLH1 protein (red). (a) 57 MLH1 sites in one SC-spread. (b) 49 MLH1 sites in one SC-spread and 11 MLH1 sites on one macrobivalent 1 (asterisk). Heterochromatin region on the bivalent 6 (HR6) is indicated. Axial elements of chromosomes are immunostained with antibodies against the SYCP3 protein (green). Chromatin stained with DAPI (blue). Bar—10 μm.
