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. 2022 Nov 24;11(4):74. doi: 10.3390/antib11040074

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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ADCC reporter assay by EpMab-37-mG_2a-f and 281-mG_2a-f in the presence of BT-474 and Capan-2 cells. (A,B) Target cells (BT-474 (A) and Capan-2 (B)) were treated with serially diluted EpMab-37-mG_2a-f and 281-mG_2a-f (mouse IgG_2a control). Then, Jurkat cells stably expressing the human FcγRIIIa receptor and an NFAT response element driving firefly luciferase were added and co-cultured. Luminescence was measured using the Bio-Glo Luciferase Assay System. ADCC, antibody-dependent cellular cytotoxicity.