Table 2.
Differentially accumulated proteins identified by ESI-LC-MS/MS.
| Protein Name | ID (Uniprot) | Organism Reference | Cellular Compartment | Fold Change Mo-CBP3-PepI vs. DMSO |
|---|---|---|---|---|
| DNA metabolism | ||||
| Endonuclease 8 | P50465 | Escherichia coli (strain K12) | Cytoplasm | 0.287 |
| Regulation of transcription | ||||
| HTH-type transcriptional regulator SutR | P77626 | Escherichia coli (strain K12) | Cytoplasm | 2.566 |
| HTH-type transcriptional regulator ArgP | P0A8S1 | Escherichia coli (strain K12) | Cytoplasm | 2.364 |
| Fimbriae Z protein | P0AEL8 | Escherichia coli (strain K12) | Cytoplasm | 0.690 |
| Transmembrane transporters | ||||
| Low affinity potassium transport system protein kup | P63183 | Escherichia coli (strain K12) | Plasma membrane | 1.704 |
| Ferrichrome outer membrane transporter/phage receptor | P06971 | Escherichia coli (strain K12) | Plasma membrane | 1.063 |
| Cation efflux system protein CusC | P77211 | Escherichia coli (strain K12) | cell outer membrane | 0.920 |
| Iron (3+)-hydroxamate import system permease protein FhuB | P06972 | Escherichia coli (strain K12) | Plasma membrane | 0.948 |
| Stress and Defense Response | ||||
| Zinc resistance-associated protein | P0AAA9 | Escherichia coli (strain K12) | Periplasm space | 1.682 |
| Chaperonin GroEL | P0A6F5 | Escherichia coli (strain K12) | Cytoplasm | 1.444 |
| Undecaprenyl-diphosphatase | Q2KX31 | Bordetella avium (strain 197N) | Plasma membrane | 1.340 |
| Peptide methionine sulfoxide reductase MsrB | P75129 | Mycoplasma pneumoniae (strain ATCC 29342/M129) | Cytoplasm | 14.104 |
| Periplasmic trehalase | Q4UZ12 | Xanthomonas campestris pv. campestris (strain 8004) | Periplasmatic space | 0.937 |
| Lon protease | P78025 | Mycoplasma pneumoniae (strain ATCC 29342/M129) | Cytoplasm | 0.342 |
| Energy and Metabolism | ||||
| 2.3-bisphosphoglycerate-dependent phosphoglycerate mutase | P62707 | Escherichia coli (strain K12) | Cytoplasm | 0.519 |
| Gamma-glutamylputrescine oxidoreductase | P37906 | Escherichia coli (strain K12) | Cytoplasm | 0.985 |
| 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase | P17109 | Escherichia coli (strain K12) | Plasma membrane | 3.486 |
| UDP-glucose 6-dehydrogenase | P76373 | Escherichia coli (strain K12) | Cytoplasm | 0.902 |
| Glycerate 3-kinase | P77364 | Escherichia coli (strain K12) | Cytoplasm | 0.745 |
| Phenylacetate-coenzyme A ligase | P76085 | Escherichia coli (strain K12) | Cytoplasm | 1.666 |
| Pantothenate kinase | P0A6I3 | Escherichia coli (strain K12) | Cytoplasm | 0.548 |
| Pyruvate kinase | P78031 | Mycoplasma pneumoniae (strain ATCC 29342/M129) | Membrane | 3.139 |
| Phosphoenolpyruvate carboxykinase (ATP) | A8AQV7 | Escherichia coli (strain K12) | Cytoplasm | 0.990 |
| Xylose isomerase | B5ZQV6 | Rhizobium leguminosarum bv. trifolii (strain WSM2304) | Cytoplasm | 1.062 |
| Bifunctional protein FolD | Q88WM8 | Lactiplantibacillus plantarum (strain ATCC BAA-793/NCIMB 8826/WCFS1) | Periplasmic space | 0.726 |
| GMP synthase (glutamine-hydrolyzing) | Q6APU2 | Desulfotalea psychrophila (strain LSv54/DSM 12343) | Cytoplasm | 4.596 |
| Sensor histidine kinase GlrK | P52101 | Escherichia coli (strain K12) | Cell inner membrane | 0.752 |
| Sensor protein PhoQ | P23837 | Escherichia coli (strain K12) | Plasma membrane | 0.823 |
| Putative ABC transporter ATP-binding protein MPN_334 | P75444 | Mycoplasma pneumoniae (strain ATCC 29342/M129) | Plasma membrane | 1.336 |
| Oligopeptide transport ATP-binding protein OppD | P75552 | Mycoplasma pneumoniae (strain ATCC 29342/M129) | Plasma membrane | 0.948 |
| Pantothenate synthetase | B8I2Z3 |
Ruminiclostridium cellulolyticum
(strain ATCC 35319/DSM 5812/JCM 6584/H10) |
Cytoplasm | 1.142 |
| Pathogenesis | ||||
| Lipopolysaccharide core biosynthesis protein RfaZ | P27241 | Escherichia coli (strain K12) | Cytoplasm | 2.038 |
| Inner membrane protein YhjD | P37642 | Escherichia coli (strain K12) | Plasma membrane | 0.362 |
| Protein Biosynthesis and Metabolism | ||||
| 50S ribosomal protein L22 | A5IYY1 | Mycoplasmopsis agalactiae | Large ribosomal subunit | 0.187 |
| 50S ribosomal protein L7/L12 | P0A466 | Aquifex aeolicus (strain VF5) | Large ribosomal subunit | 1.979 |
| Diaminopimelate decarboxylase | Q8K9C4 |
Buchnera aphidicola subsp. Schizaphis graminum (strain Sg) |
Cytoplasm | 3.546 |
| Cysteine synthase A | P0ABK5 | Escherichia coli (strain K12) | Cytoplasm | 0.596 |
| Regulation Factor and RNA Processing | ||||
| tRNA uridine 5-carboxymethylaminomethyl modification enzyme MnmG | P75221 | Mycoplasma pneumoniae (strain ATCC 29342/M129) | Cytoplasm | 0.487 |
| Elongation factor G | Q7NAV3 | Mycoplasma gallisepticum (strain R(low/passage 15/clone 2)) | Cytoplasm | 0.234 |
| Methylenetetrahydrofolate--tRNA-(uracil-5-)-methyltransferase TrmFO | A4WRQ2 | Cereibacter sphaeroides (strain ATCC 17025/ATH 2.4.3) | Cytoplasm | 0.948 |
| Cell wall organization and structural maintenance | ||||
| Cytadherence high molecular weight protein 2 | P75471 | Mycoplasma pneumoniae (strain ATCC 29342/M129) | Cytoplasm | 1.682 |
| Mgp-operon protein 3 | Q50341 | Mycoplasma pneumoniae (strain ATCC 29342/M129) | Plasma membrane | 0.654 |
| Transferase | ||||
| Glutathione S-transferase YfcF | P77544 | Escherichia coli (strain K12) | Cytoplasm | 0.273 |
| Phosphate acyltransferase | P75232 | Mycoplasma pneumoniae (strain ATCC 29342/M129) | Cytoplasm | 3.096 |
| Sensor histidine kinase HprS | P76339 | Escherichia coli (strain K12) | Plasma membrane | 0.213 |
| Unknown | ||||
| Probable cytosol aminopeptidase | P75206 | Mycoplasma pneumoniae (strain ATCC 29342/M129) | Cytoplasm | 2.097 |
| Putative acetyltransferase MPN_114 | P75448 | Mycoplasma pneumoniae (strain ATCC 29342/M129) | Unknown | 1.703 |