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. 2022 Dec 12;9(12):792. doi: 10.3390/bioengineering9120792

Figure 1.

Figure 1

The testing principle and illustration of a tumor spheroid. (a) The equipment and kit used in tumor spheroid formation, and the optical observation and images capture. (I) High content microscope with artificial intelligence-based software and microscope-associated microenvironment controller. (II) Tumor spheroid formation kit with 48-well non-adhesive U-bottom plate. (b) The illustration of tumor spheroid culture timeline. (I) Invasive spheroid. (II) Non-invasive spheroid. (c) The bright field images of NCI-H23 spheroid cultured in the microenvironment controller within 48 h with photos taken every 8 h. (d) The different layers of a spheroid. (e) The morphological characteristics of invasive and less invasive tumor spheroids in a 7-day culture. (I) Spheroid of CT-26. (II) Spheroid of HT-29.