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. 2022 Nov 23;11(12):2315. doi: 10.3390/antiox11122315

Figure 2.

Figure 2

Figure 2

Inhibition of TRPV4 decreased the expression levels of M1 phenotypic markers. (a) iNos, Il-6, Cxcl-10, and Il-1β mRNA levels in RAW26.47 cells after 100 ng/mL LPS treatment with 5 μmol/L GSK101 or 10 μmol/L HC067047 for 6 h. n = 3. One-way ANOVA with Tukey’s post-hoc test, * p < 0.05; *** p < 0.001, ****p < 0.0001. (b) Protein levels of TRPV4, COX2, and iNOS in the cells after 100ng/mL LPS treatment with 5 μmol/L GSK101 or 10 μmol/L HC067047 for 24 h. (c) Quantitation of Western blotting; n = 3. One-way (ANOVA) with Tukey’s post-hoc test, ** p < 0.01; *** p < 0.001, **** p< 0.0001. (d) Immunofluorescence analysis of iNOS in the cells after 24 h (Scale bar: 10 μm). (e) Quantitation of (d). n = 3. One-way ANOVA with Tukey’s post-hoc test, **** p< 0.0001; ns = no significance. (f) The ELISA assay was used to determine the levels of IL-6 and TNF-α in the supernatants collected after 24 h. n = 3. One-way ANOVA with Tukey’s post-hoc test, *** p < 0.001, ns = no significance. All data are shown as mean ± SD.