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. 2022 Nov 23;11(12):2315. doi: 10.3390/antiox11122315

Figure 5.

Figure 5

TRPV4 inhibition prevents M1 macrophage polarization by blocking the NLRP3 inflammasome. (a) Immunofluorescence of ASC in cells pretreated with 100ng/mL LPS or LPS + 10 μmol/L HC067047 for 6 h, without or with 5 nmol/L ATP for 1 h. (b) iNos, Il-6, Cxcl-10, and Il-1β mRNA levels in the control, LPS, LPS + HC067047, LPS + ATP, and LPS + ATP + HC067047 groups. n = 3. ANOVA with Tukey’s post-hoc test, * p < 0.05; ** p < 0.01; *** p < 0.001. (c) COX2 and iNOS protein levels in RAW264.7 cells pretreated with LPS or LPS + HC067047 for 24 h, without or with 2 nmol/L ATP for 3 h. (d) Quantitative analysis of Western blotting. n = 3. One-way ANOVA with Tukey’s post-hoc test, ** p < 0.01; *** p < 0.001. All data are shown as mean ± SD.