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. 2022 Nov 25;11(12):2329. doi: 10.3390/antiox11122329

Figure 2.

Figure 2

Nrf2 and GCL expression levels in the whole lungs of mice exposed to IQOS. (A) Analysis of nuclear Nrf2 expression in the lungs of murine cage controls, sham controls, and at various times after exposure to IQOS. The density of Nrf2 bands were measured, and the ratio of expression normalized to LaminB1 was calculated and expressed as fold-change relative to cage control mice. (B) Expression analysis of γ-GCLc in the cytoplasmic fraction of the lungs of murine cage controls, sham controls, and at various times after exposure to IQOS. The density of γ-GCLc bands were measured, and the ratio of expression normalized to β-actin was calculated and expressed as fold-change relative to cage control mice. (C) Expression analysis of γ-GCLm in the cytoplasmic fraction of murine lungs from cage controls, sham controls, and at various times after exposure to IQOS. The density of γ-GCLm bands were measured, and the ratio of expression normalized to β-actin was calculated and expressed as fold-change relative to cage control mice. N.S.: not significant. N = 3.