Skip to main content
. 2022 Nov 25;11(12):2329. doi: 10.3390/antiox11122329

Figure 3.

Figure 3

Effects of IQOS exposure on the eIF2α-ATF4 axis in murine whole-lung tissue. (A) Expression analysis of p-eIF2α and eIF2α in the cytoplasmic fractions of the lungs from murine cage controls, sham controls, and at various times after exposure to IQOS. The densities of p-eIF2α bands were measured, and the expression ratio to eIF2α was calculated and expressed as a normalized fold-change relative to cage control mice. (B) The analysis of nuclear ATF4 expression in murine lungs of cage control, sham control, and various times after exposure to IQOS is shown. The density of ATF4 bands were measured, and the expression ratio normalized to LaminB1 was calculated and expressed as fold-change relative to cage control mice. (C) Expression analysis of xCT on cell membranes in the murine lungs of cage controls, sham controls, and at various times after exposure to IQOS. The density of xCT bands were measured, and the expression ratio normalized to Pan-cadherin was calculated and expressed as fold-change relative to cage control mice. * p < 0.05 versus cage control group. N.S.: not significant. N = 3.